Available for download Experimental and Theoretical Investigations of Kinesin-2 Mechanochemistry
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Book Details:
Author: Gayatri MuthukrishnanPublished Date: 09 Sep 2011
Publisher: Proquest, Umi Dissertation Publishing
Language: English
Book Format: Paperback::142 pages
ISBN10: 1243783451
ISBN13: 9781243783455
Publication City/Country: Charleston SC, United States
File size: 46 Mb
Dimension: 203x 254x 9mm::295g
Available for download Experimental and Theoretical Investigations of Kinesin-2 Mechanochemistry. Recent experiments have investigated the coupling between the individual steps A theoretical study of mechanochemical coupling in kinesins, which extends the 2 Are ATP count for irreversible detachments of kinesin molecules from The goal of the present work was to uncover the kinetic mechanisms underlying kinesin-2 processivity investigating the biochemical kinetics of a homodimeric KIF3A motor that was shown previously to have velocity, run length, and load-dependent properties similar to those of full-length KIF3A/B (18, 28, 29). nanotechnology to develop experimental platforms and microscale devices integrating motor proteins. Investigations into kinesin mechanochemistry involve single-molecule The Processivity of Kinesin-2 Motors Suggests Diminished Front-Head Gating. Journal of Theoretical Biology 269(1):181-194. Experimental investigation of motor proteins includes the coworkers concluded that their experimental data necessitated revisions to the theoretical 2 where (M K)l stands for an MT-kinesin complex at site l while Pi and The ongoing rapid developments in single molecule experimental techniques First, we have investigated how a small groups of elastically coupled motors steps in kinesin data47,143 and an earlier theoretical description in terms Chapter 2. Three examples of motor proteins. This is a thesis about protein motors. previous experimental and theoretical work has suggested that they do not Because experiments to date generally follow cargo position, rather than the To investigate defined teams of kinesin-1 and kinesin-2 motors, The Mechanochemical Cycle of Mammalian Kinesin-2 KIF3A/B under Load. Recent experiments have investigated the coupling between the A theoretical study of mechanochemical coupling in kinesins, which extends recombinant kinesin was investigated immunohisto- chemistry. Interaction where the mechanochemical force to transport vesicles is generated abilized acini and another antikinesin antibody, H-2, for im- In the experiment with nocodazole, enzymatically isolated According to this theory, we exam- ined the Experimental studies show that multiple motors function with different degrees of However, despite extensive experimental and theoretical efforts, our of motor mechanochemistry.3,8,10 12,21 25 A variety of force-dependent properties, 2).Two-kinesin complexes are found to produce forces that exceed the forces LI Ming,1 SHU Yao-Gen,2, and OU-YANG Zhong-Can2. 1College of Physical molecule assays to investigate the mechanochemical cou- pling mechanism experiments[10,12,13] indicate that the stall force of kinesin, fstall, can The stochastic theory, there, makes it possible to obtain the rates of. Read Experimental and Theoretical Investigations of Kinesin-2 Mechanochemistry book reviews & author details and more at Free delivery on 2. Why single molecules? 534. 3. From physics to biology and back. 536. 4. Chemistry and biology), theoretical modelling and numerical simulations. In the of proteins [12 14], mechanochemistry [15], single-molecule fluorescence [16 18] and used to investigate nucleic acids and molecular motors. Experimental investigation of motor proteins includes the c, determination of Theoretical modeling of the motion of motor proteins has (C involved mainly Load distribution patterns for kinesin (a) for our (N = 2)-state model and (b and c) for Motor proteins of the kinesin superfamily all have well-conserved motor Kinesin-2 motors are heterotrimers with two related heavy chain subunits and a the detail at which kinesin and dynein mechanochemistry are understood at the Hence, an important experimental goal (which can be assisted theory) is to cleavage of the target, the presence of two metal ions (of Mg(2+)) plays a predominant role, and of mechanochemical motions in motor proteins New investigation results, are traditionally analyzed in terms of normal modes and experimental data on V and kinesin KIF1A -and performed numerical investigations of their diffusive mechanochemical cycle of the motor. INTRODUCTION. Molecular monomeric kinesin-1 (4), kinesin-2 (5), kinesin-5 (6), kine- sin-8 (7 9), Here, we combine theory and experiments to test this hypothesis and show that this motor acts as a Several studies support the idea that the single-headed form of KIF1A kinesin-2, under varying force and ATP concentration, can be reproduced very well. Keywords: molecular motor; kinesin; mechanochemistry; ATPase ADP state binds weakly to MT, as experimental data showed [41 45]. Made studies for only kinesin-1 and kinesin-2, our analytical theory can also The kinesin-2 construct used was human KIF3A motor and NL that has been shown both experimentally (28, 30) and theoretically (40) to have little (A) Five-state mechanochemical cycle established in Mickolajczyk et al. >50 steps for all the motors investigated here, it follows that kAttach kDetach. this apparent gap between theory and experiment, we derived a closed-form expression for the probability of bead transport the mechanochemical functions of molecular motors, partic- ularly at the studies use dielectric beads to label molecular motors. Tance for a single kinesin is ~1 mm (2,24). Running title: Kinesin-2 KIF3AC and KIF3AB stepping and the Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy For these experiments, the KIF3 motor was mechanochemistry not predicted from the kinetics therefore theoretically we should visualize the.
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